3D4S

Cholesterol bound form of human beta2 adrenergic receptor.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 23-ID-D
Synchrotron site	APS
Beamline	23-ID-D
Temperature [K]	77
Detector technology	CCD
Collection date	2007-12-11
Detector	MARMOSAIC 300 mm CCD
Wavelength(s)	1.03324
Spacegroup name	P 21 21 21
Unit cell lengths	40.000, 75.700, 172.730
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	20.000 - 2.800
Rwork	0.230
R-free	0.27250
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2rh1
RMSD bond length	0.010
RMSD bond angle	1.313
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX ((phenix.refine))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	3.000
High resolution limit [Å]	2.800	2.800
Number of reflections	13598
<I/σ(I)>	6.9	1.9
Completeness [%]	94.0	91
Redundancy	4.2	2.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	MESOPHASE	7	293	28% v/v PEG 400, 300mM K formate, 100mM Bis-tris propane pH 7.0, 2mM Timolol, MESOPHASE, temperature 293K
1	MESOPHASE	7	293	28% v/v PEG 400, 300mM K formate, 100mM Bis-tris propane pH 7.0, 2mM Timolol, MESOPHASE, temperature 293K