3D2W
Crystal structure of mouse TDP-43 RRM2 domain in complex with DNA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSRRC BEAMLINE BL13C1 |
| Synchrotron site | NSRRC |
| Beamline | BL13C1 |
| Temperature [K] | 123 |
| Detector technology | CCD |
| Collection date | 2008-01-23 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 1 |
| Spacegroup name | F 2 2 2 |
| Unit cell lengths | 41.283, 87.290, 125.345 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 25.460 - 1.650 |
| R-factor | 0.21031 |
| Rwork | 0.207 |
| R-free | 0.24515 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1wf0 |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.257 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | CCP4 |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 25.460 | 1.710 |
| High resolution limit [Å] | 1.650 | 1.650 |
| Rmerge | 0.058 | 0.320 |
| Number of reflections | 13854 | |
| <I/σ(I)> | 14 | 3.51 |
| Completeness [%] | 98.7 | 97 |
| Redundancy | 4.7 | 4.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.2 | 298 | Reservoir solution containing 2M (NH4)2SO4, 0.1M phosphate-citrate, the reservoir solution contained an addition of 10% glycerol, pH4.2, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | (NH4)2SO4 | ||
| 2 | 1 | 1 | phosphate-citrate | ||
| 3 | 1 | 1 | glycerol | ||
| 4 | 1 | 1 | HOH | ||
| 5 | 1 | 2 | (NH4)2SO4 | ||
| 6 | 1 | 2 | phosphate-citrate | ||
| 7 | 1 | 2 | glycerol | ||
| 8 | 1 | 2 | HOH |
