3D0E
Crystal structure of human Akt2 in complex with GSK690693
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 17-ID |
| Synchrotron site | APS |
| Beamline | 17-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | Quantum 210 |
| Wavelength(s) | 1.0000 |
| Spacegroup name | P 32 |
| Unit cell lengths | 116.489, 116.489, 45.119 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 35.670 - 2.000 |
| R-factor | 0.208 |
| Rwork | 0.206 |
| R-free | 0.24600 |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.269 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 35.670 | 50.000 | 2.070 |
| High resolution limit [Å] | 2.000 | 4.310 | 2.000 |
| Rmerge | 0.102 | 0.045 | 0.615 |
| Number of reflections | 46301 | ||
| <I/σ(I)> | 7.9 | ||
| Completeness [%] | 99.9 | 99.8 | 99.9 |
| Redundancy | 4.2 | 4.2 | 4.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 8 | 298 | 14% PEG 4K, 10% isopropanol, pH 8.0, vapor diffusion, temperature 298K |
