3CX5
Structure of complex III with bound cytochrome c in reduced state and definition of a minimal core interface for electron transfer.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-1 |
| Synchrotron site | ESRF |
| Beamline | ID23-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-07-11 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 0.9793 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 145.120, 165.090, 194.370 |
| Unit cell angles | 90.00, 104.09, 90.00 |
Refinement procedure
| Resolution | 18.990 - 1.900 |
| R-factor | 0.245 |
| Rwork | 0.245 |
| R-free | 0.26300 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | PDB entries 1KB9 and 1YCC |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.200 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | AMoRE |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 19.000 | 2.200 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Number of reflections | 660107 | |
| <I/σ(I)> | 7.9 | 2.43 |
| Completeness [%] | 89.2 | 79 |
| Redundancy | 3 | 2.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Microbatch (paraffin oil) | 7.5 | 277 | 1M Sucrose, 10% DMSO, 20mM Tris pH 7.5, 80mM NaCl, 0.05 % UM, 1 M stigmatellin, 5% PEG 4000, Microbatch (paraffin oil), temperature 277K |
