3CW2

Crystal structure of the intact archaeal translation initiation factor 2 from Sulfolobus solfataricus .

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	EMBL/DESY, HAMBURG BEAMLINE X12
Synchrotron site	EMBL/DESY, HAMBURG
Beamline	X12
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2007-02-16
Detector	MAR scanner 345 mm plate
Wavelength(s)	0.84230
Spacegroup name	P 1 21 1
Unit cell lengths	79.200, 162.920, 161.280
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	19.912 - 2.800
Rwork	0.225
R-free	0.27600
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2plf
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	19.913	2.850
High resolution limit [Å]	2.800	2.800
Rmerge	0.069
Number of reflections	95812
<I/σ(I)>	10.71
Completeness [%]	95.3	90.9
Redundancy	3.16

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.5	295	0.1M Tris HCl, 0.72M sodium formate, 9% PEG 8000, 9% PEG 1000, 1% monomethyl ether polyethylene glycol 5000 , pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 295K