3CUE
Crystal structure of a TRAPP subassembly activating the Rab Ypt1p
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-C |
| Synchrotron site | APS |
| Beamline | 24-ID-C |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-06-07 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.9795, 0.9537 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 115.120, 115.400, 290.070 |
| Unit cell angles | 90.00, 90.28, 90.00 |
Refinement procedure
| Resolution | 25.000 - 3.700 |
| R-factor | 0.265 |
| Rwork | 0.265 |
| R-free | 0.29900 |
| Structure solution method | MAD |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.840 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | SHARP |
| Refinement software | CNS (1.2) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.830 |
| High resolution limit [Å] | 3.700 | 3.700 |
| Rmerge | 0.080 | 0.368 |
| Number of reflections | 81195 | |
| <I/σ(I)> | 11.6 | 2.2 |
| Completeness [%] | 98.5 | 91.4 |
| Redundancy | 3.4 | 2.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 295 | 3-5% PEG 20,000, 0.1 M MES, 3% (w/v) sorbitol, 5 mM DTT, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 295K |
