3CR0
Wee1 kinase complex with inhibitor PD259_809
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RUH3R |
| Temperature [K] | 110 |
| Detector technology | IMAGE PLATE |
| Collection date | 2002-08-15 |
| Detector | MAR scanner 345 mm plate |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 69.659, 69.659, 157.279 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 30.000 - 2.300 |
| R-factor | 0.20757 |
| Rwork | 0.205 |
| R-free | 0.25462 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1x8b |
| RMSD bond length | 0.026 |
| RMSD bond angle | 2.279 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | REFMAC |
| Refinement software | REFMAC (5.4.0067) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 63.250 | 2.380 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.118 | 0.675 |
| Number of reflections | 17891 | |
| <I/σ(I)> | 22.8 | 2.48 |
| Completeness [%] | 99.3 | 95.1 |
| Redundancy | 8.34 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293 | HEPES, sodium chloride, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
