3CMJ
Crystal Structure of engineered Beta-Glucosidase from Soil metagenome
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PAL/PLS BEAMLINE 6C1 |
| Synchrotron site | PAL/PLS |
| Beamline | 6C1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-01-08 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 1.23986 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 70.331, 71.002, 86.978 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.000 - 1.600 |
| R-factor | 0.165 |
| Rwork | 0.163 |
| R-free | 0.19800 |
| Starting model (for MR) | PDB code 1OIM |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.341 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASES |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 1.660 |
| High resolution limit [Å] | 1.600 | 1.600 |
| Number of reflections | 54735 | |
| <I/σ(I)> | 35.57 | |
| Completeness [%] | 94.0 | |
| Redundancy | 5 | 2.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 295 | Na/K tartrate, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 295K |
