3CMI
Crystal structure of glutathione-dependent phospholipid peroxidase Hyr1 from the yeast Saccharomyces cerevisiae
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2007-10-30 |
| Detector | MAR scanner 345 mm plate |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 39.570, 64.880, 72.020 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 34.680 - 2.020 |
| R-factor | 0.22597 |
| Rwork | 0.224 |
| R-free | 0.25602 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2p5q |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.168 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 39.560 | 2.110 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.057 | 0.336 |
| Number of reflections | 12069 | |
| <I/σ(I)> | 3.6 | |
| Completeness [%] | 93.9 | 81.5 |
| Redundancy | 4.8 | 4.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.6 | 289 | 0.2M Ammonium Acetate, 0.1M Sodium Acetate trihydrate, pH4.6, 30% polyethylene glycol 4000, VAPOR DIFFUSION, HANGING DROP, temperature 289K |
