3CL2
N1 Neuraminidase N294S + Oseltamivir
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2008-01-10 |
| Detector | RIGAKU RAXIS IV++ |
| Wavelength(s) | 1.54 |
| Spacegroup name | P 6 |
| Unit cell lengths | 290.649, 290.649, 133.293 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 29.853 - 2.538 |
| R-factor | 0.24 |
| Rwork | 0.239 |
| R-free | 0.26300 |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.276 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | PHENIX ((phenix.refine)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 29.800 | 2.700 |
| High resolution limit [Å] | 2.540 | 2.600 |
| Number of reflections | 190670 | |
| Completeness [%] | 91.9 | 88.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8 | 293 | 15% PEG3350, pH 8.0, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
