3CKK
Crystal structure of human methyltransferase-like protein 1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 23-ID-B |
| Synchrotron site | APS |
| Beamline | 23-ID-B |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-03-05 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 1.03310 |
| Spacegroup name | P 65 |
| Unit cell lengths | 90.316, 90.316, 54.940 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 50.000 - 1.550 |
| R-factor | 0.19962 |
| Rwork | 0.199 |
| R-free | 0.21835 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2vdu |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.276 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 100.000 | 1.610 |
| High resolution limit [Å] | 1.550 | 1.550 |
| Rmerge | 0.068 | 0.596 |
| Number of reflections | 36869 | |
| <I/σ(I)> | 13.2 | 1.78 |
| Completeness [%] | 99.1 | 91.3 |
| Redundancy | 11.2 | 4.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 297 | 2.0 M Ammonium sulfate, 2% PEG 400, 0.1 M Na HEPES, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 297K |
