3CH5
The crystal structure of the RanGDP-Nup153ZnF2 complex
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X10SA |
Synchrotron site | SLS |
Beamline | X10SA |
Temperature [K] | 100 |
Detector technology | CCD |
Detector | MAR CCD 165 mm |
Wavelength(s) | 1.2726 |
Spacegroup name | I 2 2 2 |
Unit cell lengths | 69.700, 75.350, 123.050 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 19.760 - 2.100 |
R-factor | 0.19821 |
Rwork | 0.196 |
R-free | 0.23443 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | RanGDP |
RMSD bond length | 0.014 |
RMSD bond angle | 1.674 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | MOLREP |
Refinement software | REFMAC (5.2) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 19.760 | 2.200 |
High resolution limit [Å] | 2.100 | 2.100 |
Rmerge | 0.310 | |
Number of reflections | 18026 | |
<I/σ(I)> | 16.1 | 6.31 |
Completeness [%] | 98.2 | 99.9 |
Redundancy | 4.5 | 4.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 293 | 2 M ammonium sulfate, 0.1 M Bis Tris, pH 5.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |