3CH4
The Crystal Structure of Human Phosphomavelonate Kinase At 1.8 A Resolution
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2007-11-29 |
| Detector | MAR scanner 345 mm plate |
| Wavelength(s) | 1.5418 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 93.140, 32.625, 61.786 |
| Unit cell angles | 90.00, 111.01, 90.00 |
Refinement procedure
| Resolution | 8.000 - 1.760 |
| R-factor | 0.199 |
| Rwork | 0.199 |
| R-free | 0.22100 |
| Structure solution method | SAD |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.523 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SHARP |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 8.000 | 1.800 |
| High resolution limit [Å] | 1.760 | 1.760 |
| Rmerge | 0.055 | 0.442 |
| Number of reflections | 16987 | |
| <I/σ(I)> | 16.9 | 5.4 |
| Completeness [%] | 99.4 | 98.2 |
| Redundancy | 9.8 | 9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.6 | 288 | 15-20% (v/v) Pentaerythritol ethoxylate (15/4 EO/OH), 15% (v/v) MPD, 0.1M Hepes, pH 7.6, VAPOR DIFFUSION, SITTING DROP, temperature 288K |
