3CGU
Crystal Structure of unliganded Argos
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.2.1 |
| Synchrotron site | ALS |
| Beamline | 8.2.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-11-09 |
| Detector | ADSC QUANTUM 315r |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 113.583, 64.246, 72.530 |
| Unit cell angles | 90.00, 101.59, 90.00 |
Refinement procedure
| Resolution | 35.530 - 2.510 |
| R-factor | 0.258 |
| Rwork | 0.256 |
| R-free | 0.29900 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3c9a |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.029 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.580 |
| High resolution limit [Å] | 2.510 | 2.510 |
| Rmerge | 0.055 | 0.364 |
| Number of reflections | 15720 | |
| <I/σ(I)> | 15.1 | 2.7 |
| Completeness [%] | 93.9 | 80.9 |
| Redundancy | 3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 4.5 | 291 | 10-20% PEG3350, 0.1M NaAcetate pH 4.5, 0.2M ammonium sulphate, VAPOR DIFFUSION, HANGING DROP, temperature 291K, pH 4.50 |
