3CF6
Structure of Epac2 in complex with cyclic-AMP and Rap
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-1 |
| Synchrotron site | ESRF |
| Beamline | ID23-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-10-07 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.9732 |
| Spacegroup name | I 21 21 21 |
| Unit cell lengths | 125.367, 149.025, 225.291 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 48.560 - 2.200 |
| R-factor | 0.24446 |
| Rwork | 0.243 |
| R-free | 0.26503 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2byv pdb entry 1BKD |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.418 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.300 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Rmerge | 0.084 | 0.490 |
| Number of reflections | 102111 | |
| <I/σ(I)> | 2.96 | |
| Completeness [%] | 95.6 | 87.3 |
| Redundancy | 3.62 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.6 | 277 | 0.4M (NH4)2SO4, 1.2M Li2SO4, 0.1M citrate, pH 5.6, VAPOR DIFFUSION, SITTING DROP, temperature 277K |
