3CF6
Structure of Epac2 in complex with cyclic-AMP and Rap
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID23-1 |
Synchrotron site | ESRF |
Beamline | ID23-1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2007-10-07 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.9732 |
Spacegroup name | I 21 21 21 |
Unit cell lengths | 125.367, 149.025, 225.291 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 48.560 - 2.200 |
R-factor | 0.24446 |
Rwork | 0.243 |
R-free | 0.26503 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2byv pdb entry 1BKD |
RMSD bond length | 0.012 |
RMSD bond angle | 1.418 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | MOLREP |
Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.300 |
High resolution limit [Å] | 2.200 | 2.200 |
Rmerge | 0.084 | 0.490 |
Number of reflections | 102111 | |
<I/σ(I)> | 2.96 | |
Completeness [%] | 95.6 | 87.3 |
Redundancy | 3.62 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 5.6 | 277 | 0.4M (NH4)2SO4, 1.2M Li2SO4, 0.1M citrate, pH 5.6, VAPOR DIFFUSION, SITTING DROP, temperature 277K |