3CDG

Human CD94/NKG2A in complex with HLA-E

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X25
Synchrotron site	NSLS
Beamline	X25
Temperature [K]	100
Detector technology	CCD
Collection date	2007-10-02
Detector	ADSC QUANTUM 315
Spacegroup name	I 41 3 2
Unit cell lengths	345.201, 345.201, 345.201
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	50.000 - 3.400
R-factor	0.25
Rwork	0.249
R-free	0.27900
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	pbd code 3bdw 3bze
RMSD bond length	0.006
RMSD bond angle	0.944
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000
High resolution limit [Å]	3.400	3.400
Number of reflections	48105
<I/σ(I)>		4.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.9	291	Ammonium sulfate, pH 7.9, VAPOR DIFFUSION, HANGING DROP, temperature 291K