3CD3
Crystal structure of phosphorylated human feline sarcoma viral oncogene homologue (v-FES) in complex with staurosporine and a consensus peptide
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU FR-E+ SUPERBRIGHT |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2008-01-25 |
| Detector | RIGAKU RAXIS |
| Wavelength(s) | 1.54178 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 35.451, 76.905, 150.632 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 15.347 - 1.980 |
| R-factor | 0.188 |
| Rwork | 0.185 |
| R-free | 0.24700 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3bkb |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.603 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 15.347 | 2.090 |
| High resolution limit [Å] | 1.980 | 1.980 |
| Rmerge | 0.082 | 0.472 |
| Number of reflections | 29000 | |
| <I/σ(I)> | 10.2 | 2.3 |
| Completeness [%] | 97.8 | 100 |
| Redundancy | 3.5 | 3.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 277 | 30% PEG MME 2000, 0.15M Sodium bromide, pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 277K |
