3CBB
Crystal Structure of Hepatocyte Nuclear Factor 4alpha in complex with DNA: Diabetes Gene Product
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-BM |
| Synchrotron site | APS |
| Beamline | 22-BM |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-08-20 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 0.92017 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 121.628, 35.425, 70.985 |
| Unit cell angles | 90.00, 119.36, 90.00 |
Refinement procedure
| Resolution | 20.000 - 2.000 |
| Rwork | 0.213 |
| R-free | 0.25200 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.031 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 2.070 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.064 | 0.304 |
| Number of reflections | 17094 | |
| <I/σ(I)> | 12.1 | |
| Completeness [%] | 93.9 | 72.4 |
| Redundancy | 5.6 | 3.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.8 | 298 | 26% PEG 4000, 80mM Magnesium acetate, 50mM Sodium citrate, pH 4.8, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | PEG 4000 | ||
| 2 | 1 | 1 | Magnesium acetate | ||
| 3 | 1 | 1 | Sodium citrate | ||
| 4 | 1 | 2 | PEG 4000 | ||
| 5 | 1 | 2 | Magnesium acetate | ||
| 6 | 1 | 2 | Sodium citrate |
