3CB2

Crystal structure of human gamma-tubulin bound to GDP

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 8.2.1
Synchrotron site	ALS
Beamline	8.2.1
Temperature [K]	100
Detector technology	CCD
Collection date	2005-12-06
Detector	ADSC QUANTUM 315
Wavelength(s)	1.0
Spacegroup name	P 1 21 1
Unit cell lengths	51.744, 108.755, 83.347
Unit cell angles	90.00, 96.33, 90.00

Refinement procedure

Resolution	82.760 - 2.303
R-factor	0.18908
Rwork	0.187
R-free	0.23786
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	PDB 1Z5V stripped of sidechains and ligands
RMSD bond length	0.008
RMSD bond angle	1.149
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	REFMAC (5.2)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	82.760	2.380
High resolution limit [Å]	2.303	2.303
Number of reflections	38587
Completeness [%]	98.9	97

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	7.2	298	top solution: 83 mM HEPES pH 7.2, 1.2M KCl, 20% PEG6000, 1mM GDP; reservoir: 83mM HEPES pH 7.2, 0.6M KCl, 20% PEG6000, 1 mM GDP. Protein was mixed 1:1 or 1:2 with top solution, and equilibrated over the reservoir solution., VAPOR DIFFUSION, temperature 298K