3C9A
High Resolution Crystal Structure of Argos bound to the EGF domain of Spitz
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 23-ID-D |
| Synchrotron site | APS |
| Beamline | 23-ID-D |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-04-05 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 0.97928, 0.91961, 0.91987, 0.89 |
| Spacegroup name | P 1 |
| Unit cell lengths | 49.991, 51.275, 69.985 |
| Unit cell angles | 84.23, 74.82, 75.67 |
Refinement procedure
| Resolution | 40.760 - 1.600 |
| R-factor | 0.198 |
| Rwork | 0.195 |
| R-free | 0.24000 |
| Structure solution method | MAD |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.357 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | SHELXCD |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.660 |
| High resolution limit [Å] | 1.600 | 1.600 |
| Rmerge | 0.065 | 0.355 |
| Number of reflections | 75754 | |
| <I/σ(I)> | 11.3 | 2.64 |
| Completeness [%] | 94.6 | 71.4 |
| Redundancy | 3.7 | 2.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7 | 294 | 0.1M HEPES pH 7.0, 24% ethylene glycol,(0.1-1%) PEG20000, VAPOR DIFFUSION, HANGING DROP, temperature 294K, pH 7.00 |
