3C6W
Crystal structure of the ING5 PHD finger in complex with H3K4me3 peptide
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 4.2.2 |
| Synchrotron site | ALS |
| Beamline | 4.2.2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-04-22 |
| Detector | NOIR-1 |
| Wavelength(s) | 1.2574, 1.28304, 1.28264 |
| Spacegroup name | P 43 |
| Unit cell lengths | 68.140, 68.140, 28.250 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 34.000 - 1.750 |
| R-factor | 0.186 |
| Rwork | 0.186 |
| R-free | 0.19900 |
| Structure solution method | MAD |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.337 |
| Data reduction software | d*TREK |
| Data scaling software | d*TREK |
| Phasing software | SOLVE |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 34.000 | 1.810 |
| High resolution limit [Å] | 1.750 | 1.750 |
| Rmerge | 0.070 | 0.219 |
| Number of reflections | 13176 | |
| <I/σ(I)> | 17.7 | 6.2 |
| Redundancy | 6.95 | 5.23 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 291.15 | 1.6 M Sodium Citrate, pH 6.5, VAPOR DIFFUSION, SITTING DROP, temperature 291.15K |
