3C6K
Crystal structure of human spermine synthase in complex with spermidine and 5-methylthioadenosine
Replaces: 2QFMExperimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU FR-E+ DW |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2006-07-12 |
| Detector | RIGAKU |
| Wavelength(s) | 1.54178 |
| Spacegroup name | P 1 |
| Unit cell lengths | 43.385, 74.032, 143.226 |
| Unit cell angles | 94.23, 92.88, 107.07 |
Refinement procedure
| Resolution | 40.560 - 1.950 |
| R-factor | 0.2007 |
| Rwork | 0.198 |
| R-free | 0.25744 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2o06 |
| RMSD bond length | 0.017 |
| RMSD bond angle | 1.667 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 50.000 |
| High resolution limit [Å] | 1.950 |
| Number of reflections | 115647 |
| Completeness [%] | 94.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 300 | 12% PEG 20000, 0.1 M MES pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 300K |
