3C5X
Crystal structure of the precursor membrane protein- envelope protein heterodimer from the dengue 2 virus at low pH
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 23-ID-D |
Synchrotron site | APS |
Beamline | 23-ID-D |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2007-06-17 |
Detector | MARMOSAIC 300 mm CCD |
Wavelength(s) | 1.03 |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 71.209, 108.595, 108.738 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 50.000 - 2.200 |
R-factor | 0.2555 |
Rwork | 0.253 |
R-free | 0.27530 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1tg8 |
RMSD bond length | 0.027 |
RMSD bond angle | 1.727 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | CNS |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.280 |
High resolution limit [Å] | 2.200 | 2.200 |
Rmerge | 0.056 | 0.408 |
Number of reflections | 42500 | |
<I/σ(I)> | 30.3 | 3.8 |
Completeness [%] | 98.5 | 92.9 |
Redundancy | 6.8 | 5.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 293 | Bis-Tris 0.1M pH5.5, PEG 3350 12%, VAPOR DIFFUSION, HANGING DROP, temperature 293K |