3C5W
Complex between PP2A-specific methylesterase PME-1 and PP2A core enzyme
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X29A |
| Synchrotron site | NSLS |
| Beamline | X29A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-09-10 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1.0809 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 129.292, 54.839, 125.146 |
| Unit cell angles | 90.00, 110.87, 90.00 |
Refinement procedure
| Resolution | 50.000 - 2.800 |
| R-factor | 0.19844 |
| Rwork | 0.195 |
| R-free | 0.26338 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | PP2A A subunit PP2A C subunit PME-1 monomer |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.087 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.3.0038) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 100.000 | 2.900 |
| High resolution limit [Å] | 2.800 | 2.800 |
| Number of reflections | 20623 | |
| Completeness [%] | 98.6 | 95.7 |
| Redundancy | 3 | 2.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 290 | 25% w/v PEG3350, 100 mM ammonium citrate, and 5 mM DTT, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 290K |
