3C5H

Crystal structure of the Ras homolog domain of human GRLF1 (p190RhoGAP)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 23-ID-B
Synchrotron site	APS
Beamline	23-ID-B
Temperature [K]	100
Detector technology	CCD
Collection date	2007-11-29
Detector	MARMOSAIC 300 mm CCD
Wavelength(s)	0.97926
Spacegroup name	I 4
Unit cell lengths	108.959, 108.959, 51.319
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	19.260 - 1.800
R-factor	0.207
Rwork	0.205
R-free	0.24700
Structure solution method	MAD
RMSD bond length	0.017
RMSD bond angle	1.465
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	SOLVE (2.13)
Refinement software	REFMAC (refmac_5.3.0037)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	1.860
High resolution limit [Å]	1.800	3.880	1.800
Rmerge	0.075	0.045	0.456
Number of reflections	26661
<I/σ(I)>	10.9
Completeness [%]	95.1	100	61.8
Redundancy	6.7	7.4	2.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1				Native crystal, used for model refinement: 26% PEG 4000, 0.1M Tris-HCl, 0.2M Magnesium chloride, 0.1mM DTT, pH 8.0, VAPOR DIFFUSION, SITTING DROP, temperature 291K
1				Native crystal, used for model refinement: 26% PEG 4000, 0.1M Tris-HCl, 0.2M Magnesium chloride, 0.1mM DTT, pH 8.0, VAPOR DIFFUSION, SITTING DROP, temperature 291K