3C5G
Structure of a ternary complex of the R517K Pol lambda mutant
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 HF |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-11-23 |
| Detector | RIGAKU SATURN 92 |
| Wavelength(s) | 1.54178 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 94.335, 150.819, 85.822 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 50.000 - 2.200 |
| Rwork | 0.201 |
| R-free | 0.23600 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 2.280 |
| High resolution limit [Å] | 2.200 | 4.740 | 2.200 |
| Rmerge | 0.091 | 0.059 | 0.410 |
| Number of reflections | 60372 | ||
| <I/σ(I)> | 11.1 | ||
| Completeness [%] | 96.8 | 99.8 | 89.6 |
| Redundancy | 5.7 | 8.8 | 2.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 277 | 0.1M KCl, 10 mM MgCl2, 50 mM Tris-HCl, pH 8.5 and 30% PEG 400, VAPOR DIFFUSION, SITTING DROP, temperature 277K |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | KCl | ||
| 2 | 1 | 1 | MgCl2 | ||
| 3 | 1 | 1 | Tris-HCl | ||
| 4 | 1 | 1 | PEG 40 | ||
| 5 | 1 | 2 | KCl | ||
| 6 | 1 | 2 | MgCl2 | ||
| 7 | 1 | 2 | Tris-HCl | ||
| 8 | 1 | 2 | PEG 40 |
