Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.2.1 |
| Synchrotron site | ALS |
| Beamline | 8.2.1 |
| Wavelength(s) | 1.5000 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 73.890, 81.712, 73.583 |
| Unit cell angles | 90.00, 99.51, 90.00 |
Refinement procedure
| Resolution | 50.000 - 2.500 |
| R-factor | 0.226 |
| Rwork | 0.223 |
| R-free | 0.27700 |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.094 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 2.590 |
| High resolution limit [Å] | 2.500 | 5.380 | 2.500 |
| Rmerge | 0.055 | 0.047 | 0.277 |
| Number of reflections | 29547 | ||
| <I/σ(I)> | 14.2 | ||
| Completeness [%] | 97.7 | 99.4 | 82.2 |
| Redundancy | 6.9 | 7.3 | 5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6.5 | 2.0 M Ammonium Sulfate, 100 mM MES, 20 mM NaCl, 10 mM CaCl2, pH 6.5 |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | Ammonium Sulfate | ||
| 2 | 1 | 1 | MES | ||
| 3 | 1 | 1 | NaCl | ||
| 4 | 1 | 1 | CaCl2 | ||
| 5 | 1 | 2 | Ammonium Sulfate | ||
| 6 | 1 | 2 | MES | ||
| 7 | 1 | 2 | NaCl | ||
| 8 | 1 | 2 | CaCl2 |
