3C09
Crystal structure the Fab fragment of matuzumab (Fab72000) in complex with domain III of the extracellular region of EGFR
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X06SA |
Synchrotron site | SLS |
Beamline | X06SA |
Temperature [K] | 100 |
Detector technology | CCD |
Detector | MAR CCD 130 mm |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 141.073, 205.035, 81.577 |
Unit cell angles | 90.00, 117.49, 90.00 |
Refinement procedure
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 50.000 | 3.310 |
High resolution limit [Å] | 3.200 | 6.890 | 3.200 |
Rmerge | 0.118 | 0.048 | 0.354 |
Number of reflections | 33886 | ||
<I/σ(I)> | 8.3 | ||
Completeness [%] | 99.7 | 99.3 | 98.7 |
Redundancy | 3.5 | 3.6 | 3.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 6 | 298 | 16% PEG 3350, 1M NaCl, pH 6.0, vapor diffusion, temperature 298K |