3C09

Crystal structure the Fab fragment of matuzumab (Fab72000) in complex with domain III of the extracellular region of EGFR

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06SA
Synchrotron site	SLS
Beamline	X06SA
Temperature [K]	100
Detector technology	CCD
Detector	MAR CCD 130 mm
Spacegroup name	C 1 2 1
Unit cell lengths	141.073, 205.035, 81.577
Unit cell angles	90.00, 117.49, 90.00

Refinement procedure

Resolution	36.560 - 3.200
R-factor	0.245
Rwork	0.242
R-free	0.29900
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1yy9 3c08
RMSD bond length	0.010
RMSD bond angle	1.333
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	PHASER
Refinement software	REFMAC (5.2.0019)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	3.310
High resolution limit [Å]	3.200	6.890	3.200
Rmerge	0.118	0.048	0.354
Number of reflections	33886
<I/σ(I)>	8.3
Completeness [%]	99.7	99.3	98.7
Redundancy	3.5	3.6	3.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	6	298	16% PEG 3350, 1M NaCl, pH 6.0, vapor diffusion, temperature 298K