3BUM
Crystal structure of c-Cbl-TKB domain complexed with its binding motif in Sprouty2
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X29A |
| Synchrotron site | NSLS |
| Beamline | X29A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-09-21 |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 6 |
| Unit cell lengths | 122.256, 122.256, 54.707 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 20.000 - 2.000 |
| Rwork | 0.224 |
| R-free | 0.26300 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2cbl |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.236 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | CNS |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 2.050 |
| High resolution limit [Å] | 1.980 | 4.260 | 1.980 |
| Rmerge | 0.083 | 0.052 | 0.346 |
| Number of reflections | 32750 | ||
| <I/σ(I)> | 9.9 | ||
| Completeness [%] | 99.9 | 100 | 99.1 |
| Redundancy | 16.2 | 21.1 | 8.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 298 | ammonium sulfate, PEG3350, pH7.5, vapor diffusion, hanging drop, temperature 298K, VAPOR DIFFUSION, HANGING DROP |
