3BSZ

Crystal structure of the transthyretin-retinol binding protein-Fab complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ELETTRA BEAMLINE 5.2R
Synchrotron site	ELETTRA
Beamline	5.2R
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	1997-10-21
Detector	MAR scanner 180 mm plate
Wavelength(s)	1.3
Spacegroup name	C 2 2 2
Unit cell lengths	159.620, 223.180, 121.430
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	15.720 - 3.380
R-factor	0.239
Rwork	0.239
R-free	0.31200
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1rbp 1f41
RMSD bond length	0.010
RMSD bond angle	1.500
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	AMoRE
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	55.000	3.510
High resolution limit [Å]	3.360	3.360
Rmerge	0.160	0.340
Number of reflections	25746
<I/σ(I)>	3.3	1.9
Completeness [%]	84.4	77.7
Redundancy	3	2.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5	295	2.35M ammonium phosphate, 10mM sodium citrate, 10mM beta-mercaptoethanol, pH 5.0, VAPOR DIFFUSION, SITTING DROP, temperature 295K