3BS9

X-ray structure of human TIA-1 RRM2

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	BRUKER AXS MICROSTAR
Temperature [K]	200
Detector technology	CCD
Collection date	2006-08-16
Detector	BRUKER PROTEUM
Spacegroup name	P 61
Unit cell lengths	56.500, 56.500, 76.700
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	20.000 - 1.950
Rwork	0.216
R-free	0.24540
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	N-terminal RRM of poly-A binding protein PDB entry 1CVJ
Data reduction software	PROTEUM PLUS (PLUS)
Data scaling software	PROTEUM PLUS (PLUS)
Phasing software	PHASER
Refinement software	CNS (1.2)

Data quality characteristics

	Overall
Low resolution limit [Å]	24.460
High resolution limit [Å]	1.950
Number of reflections	10137
Completeness [%]	99.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8	295	1:1 mixture of protein with 15% polyethylene glycol 3350, 0.5 M KI, 0.1 M Tris, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 295K