3BPU
Crystal structure of the 3rd PDZ domain of human membrane associated guanylate kinase, C677S and C709S double mutant
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-12-08 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.97912 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 32.472, 61.578, 80.113 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 28.740 - 1.600 |
| R-factor | 0.20418 |
| Rwork | 0.202 |
| R-free | 0.25112 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | PDB entries 1UJV 1g9o |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.597 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.4.0066) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.060 | 1.690 |
| High resolution limit [Å] | 1.600 | 1.600 |
| Rmerge | 0.074 | 0.898 |
| Number of reflections | 10446 | |
| <I/σ(I)> | 17.1 | 2.2 |
| Completeness [%] | 99.9 | 99.7 |
| Redundancy | 7.1 | 7.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.2 | 293 | 0.05M Zn acetate pH 6.2, 30% PEG 3350, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
