3BM3
Restriction endonuclease PspGI-substrate DNA complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | MPG/DESY, HAMBURG BEAMLINE BW6 |
| Synchrotron site | MPG/DESY, HAMBURG |
| Beamline | BW6 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-08-16 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.976 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 46.884, 96.060, 127.069 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 19.380 - 1.700 |
| R-factor | 0.171 |
| Rwork | 0.170 |
| R-free | 0.19240 |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.369 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SHELXD |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 1.720 |
| High resolution limit [Å] | 1.700 | 1.700 |
| Number of reflections | 63138 | |
| <I/σ(I)> | 21.3 | 3.2 |
| Completeness [%] | 98.7 | 98 |
| Redundancy | 3.3 | 3.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 4 | 294 | 20% MPD, 0.1 M citric acid, 0.1 M cobalt (II) chloride as an additive, pH 4.0, VAPOR DIFFUSION, SITTING DROP, temperature 294K |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | MPD | ||
| 2 | 1 | 1 | citric acid | ||
| 3 | 1 | 1 | cobalt (II) chloride | ||
| 4 | 1 | 2 | MPD | ||
| 5 | 1 | 2 | citric acid | ||
| 6 | 1 | 2 | cobalt (II) chloride |
