3BK3

Crystal structure of the complex of BMP-2 and the first Von Willebrand domain type C of Crossveinless-2

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	BESSY BEAMLINE 14.2
Synchrotron site	BESSY
Beamline	14.2
Temperature [K]	100
Detector technology	CCD
Collection date	2006-12-15
Detector	MAR CCD 165 mm
Wavelength(s)	0.9799, 0.9796, 0.9079
Spacegroup name	I 41
Unit cell lengths	83.750, 83.750, 139.850
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	40.000 - 2.700
R-factor	0.205
Rwork	0.203
R-free	0.24500
Structure solution method	MAD
RMSD bond length	0.013
RMSD bond angle	1.603
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	SHARP
Refinement software	REFMAC

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	40.700	2.800
High resolution limit [Å]	2.700	2.700
Number of reflections	13191
<I/σ(I)>	8.9	3.3
Completeness [%]	99.7	99.7
Redundancy	3.3	3.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	294	2.2M ammonium phosphate, 0.1M Tris pH 7.5, 8% glycerol, 5% sucrose, VAPOR DIFFUSION, HANGING DROP, temperature 294K