3BK1
Crystal Structure Analysis of RNase J
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID14-4 |
Synchrotron site | ESRF |
Beamline | ID14-4 |
Temperature [K] | 93 |
Detector technology | CCD |
Collection date | 2006-12-09 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.9794 |
Spacegroup name | F 2 2 2 |
Unit cell lengths | 97.830, 117.620, 230.490 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 53.750 - 2.330 |
R-factor | 0.197 |
Rwork | 0.197 |
R-free | 0.22000 |
Structure solution method | SAD |
RMSD bond length | 0.006 |
RMSD bond angle | 1.300 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | SHELX |
Refinement software | CNS (1.2) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 59.000 | 2.400 |
High resolution limit [Å] | 2.330 | 2.330 |
Rmerge | 0.060 | 0.171 |
Number of reflections | 28236 | |
<I/σ(I)> | 30.53 | 10.22 |
Completeness [%] | 98.7 | 88.5 |
Redundancy | 13.5 | 7.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 293 | 2-4% PEG 4K, 0.02M ammonium sulfate, 0.1M Na-MES, pH5.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |