3BGS
Structure of human purine nucleoside phosphorylase with L-DADMe-ImmH and phosphate
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X29A |
| Synchrotron site | NSLS |
| Beamline | X29A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-11-05 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.9793 |
| Spacegroup name | H 3 2 |
| Unit cell lengths | 142.801, 142.801, 167.348 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 99.500 - 2.099 |
| R-factor | 0.219 |
| Rwork | 0.218 |
| R-free | 0.24900 |
| Starting model (for MR) | 1rr6 |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.639 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 99.500 | 2.180 |
| High resolution limit [Å] | 2.099 | 2.099 |
| Rmerge | 0.074 | 0.714 |
| Number of reflections | 38397 | |
| <I/σ(I)> | 13.6 | 2.5 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 12.5 | 7.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5 | 291 | 0.1 M Sodium citrate pH 5.0, 2.3 M ammonium dihydrogenphosphate, Vapor diffusion, sitting drop, temperature 291K |
