3BFU
Structure of the ligand-binding core of GluR2 in complex with the agonist (R)-TDPA at 1.95 A resolution
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | BESSY BEAMLINE 14.1 |
| Synchrotron site | BESSY |
| Beamline | 14.1 |
| Temperature [K] | 110 |
| Detector technology | CCD |
| Collection date | 2003-06-27 |
| Detector | MAR CCD 165 mm |
| Wavelength(s) | 0.8122 |
| Spacegroup name | P 1 2 1 |
| Unit cell lengths | 104.750, 47.520, 124.330 |
| Unit cell angles | 90.00, 113.54, 90.00 |
Refinement procedure
| Resolution | 96.230 - 1.950 |
| Rwork | 0.200 |
| R-free | 0.23300 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1mqd |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.200 |
| Data scaling software | XSCALE |
| Phasing software | MOLREP |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 96.230 | 2.060 |
| High resolution limit [Å] | 1.950 | 1.950 |
| Number of reflections | 82554 | |
| <I/σ(I)> | 10.8 | 3.6 |
| Completeness [%] | 99.9 | 99.7 |
| Redundancy | 5.2 | 5.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 279 | PEG 2000, cacodylate, ammonium sulfate, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 279K |
