3BEU
Na+-Dependent Allostery Mediates Coagulation Factor Protease Active Site Selectivity
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 14-BM-C |
| Synchrotron site | APS |
| Beamline | 14-BM-C |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-08-19 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.90020 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 41.786, 72.433, 122.492 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 10.000 - 1.050 |
| R-factor | 0.1209 |
| Rwork | 0.119 |
| R-free | 0.14620 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1os8 |
| RMSD bond length | 0.015 |
| RMSD bond angle | 0.033 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | SHELXL-97 |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.070 |
| High resolution limit [Å] | 1.050 | 1.050 |
| Rmerge | 0.066 | 0.384 |
| Number of reflections | 173357 | |
| <I/σ(I)> | 26.8 | 3.6 |
| Completeness [%] | 99.6 | 94.5 |
| Redundancy | 8.7 | 6.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | 298 | 1.6 M ammonium sulfate, 10 mM calcium acetate, 50 mM sodium chloride, 10 mM benzamidine, pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
