3BBP

Rab6-GTP:GCC185 Rab binding domain complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL7-1
Synchrotron site	SSRL
Beamline	BL7-1
Temperature [K]	298
Detector technology	CCD
Collection date	2007-05-04
Detector	ADSC QUANTUM 315
Wavelength(s)	0.9785
Spacegroup name	P 64 2 2
Unit cell lengths	168.599, 168.599, 168.955
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	50.000 - 3.000
R-factor	0.228
Rwork	0.228
R-free	0.26800
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2gil
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	PHASER
Refinement software	REFMAC (5.1.24)

Data quality characteristics

	Overall
Low resolution limit [Å]	50.000
High resolution limit [Å]	3.000
Rmerge	0.250
Number of reflections	28379
<I/σ(I)>	10.1
Completeness [%]	99.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	298	20% PEG 3350, 200mM CNNaS, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K
1	VAPOR DIFFUSION, HANGING DROP	7.5	298	20% PEG 3350, 200mM CNNaS, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K