3B9U
Crystal structure of L26N/D28N/H93G mutant of Human acidic fibroblast growth factor
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X25 |
| Synchrotron site | NSLS |
| Beamline | X25 |
| Temperature [K] | 103 |
| Detector technology | CCD |
| Collection date | 2006-09-28 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 33.389, 44.190, 47.957 |
| Unit cell angles | 90.00, 100.45, 90.00 |
Refinement procedure
| Resolution | 32.840 - 1.550 |
| R-factor | 0.191 |
| Rwork | 0.191 |
| R-free | 0.20000 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1k5u |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.400 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 32.840 | 1.610 |
| High resolution limit [Å] | 1.550 | 1.550 |
| Rmerge | 0.062 | 0.231 |
| Number of reflections | 17570 | |
| <I/σ(I)> | 41.87 | 3.02 |
| Completeness [%] | 89.7 | 75.4 |
| Redundancy | 4.4 | 1.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 298.15 | PEG4000, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 298.15K |
