3B7W
Crystal structure of human acyl-CoA synthetase medium-chain family member 2A, with L64P mutation
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU FR-E+ SUPERBRIGHT |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2007-09-25 |
| Detector | RIGAKU RAXIS HTC |
| Wavelength(s) | 1.5418 |
| Spacegroup name | I 2 2 2 |
| Unit cell lengths | 98.426, 109.173, 176.926 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 50.000 - 2.000 |
| R-factor | 0.17136 |
| Rwork | 0.169 |
| R-free | 0.21385 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1pg3 |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.446 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.3.0040) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.570 | 2.110 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.077 | 0.872 |
| Number of reflections | 60580 | |
| <I/σ(I)> | 12.8 | 2 |
| Completeness [%] | 98.9 | 98.2 |
| Redundancy | 4.6 | 4.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 293 | 0.1M HEPES pH 7.5, 1.4M Na citrate, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
