3B7L
Human farnesyl diphosphate synthase complexed with MG and minodronate
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2007-08-30 |
| Detector | RIGAKU RAXIS HTC |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 110.922, 110.922, 67.308 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.210 - 1.950 |
| R-factor | 0.17078 |
| Rwork | 0.169 |
| R-free | 0.20220 |
| Structure solution method | FOURIER SYNTHESIS |
| Starting model (for MR) | 1yv5 |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.470 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.440 | 2.060 |
| High resolution limit [Å] | 1.950 | 1.950 |
| Rmerge | 0.065 | 0.606 |
| Number of reflections | 30937 | |
| <I/σ(I)> | 20.6 | 2.3 |
| Completeness [%] | 99.2 | 95.3 |
| Redundancy | 6.8 | 5.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 293 | 0.2M NH4Cl, 20% PEG 6000, 10% Ethylene glycol, pH 7.0, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
