3B4V
X-Ray structure of Activin in complex with FSTL3
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-BM |
| Synchrotron site | APS |
| Beamline | 19-BM |
| Temperature [K] | 100 |
| Spacegroup name | P 1 |
| Unit cell lengths | 63.613, 71.384, 100.203 |
| Unit cell angles | 98.55, 90.64, 90.11 |
Refinement procedure
| Resolution | 31.780 - 2.480 |
| R-factor | 0.22926 |
| Rwork | 0.227 |
| R-free | 0.27884 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.083 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 33.020 | 2.590 |
| High resolution limit [Å] | 2.475 | 2.480 |
| Rmerge | 0.085 | 0.448 |
| Number of reflections | 61295 | |
| <I/σ(I)> | 15 | 2.3 |
| Completeness [%] | 98.6 | 98.3 |
| Redundancy | 4.2 | 3.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 298 | PEG 3350, ammonium sulfate, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
