3B0P
tRNA-dihydrouridine synthase from Thermus thermophilus
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PHOTON FACTORY BEAMLINE AR-NE3A |
Synchrotron site | Photon Factory |
Beamline | AR-NE3A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2010-10-09 |
Detector | ADSC QUANTUM 270 |
Wavelength(s) | 1.000 |
Spacegroup name | P 1 |
Unit cell lengths | 42.010, 60.190, 67.000 |
Unit cell angles | 76.22, 88.67, 70.67 |
Refinement procedure
Resolution | 19.775 - 1.700 |
R-factor | 0.1701 |
Rwork | 0.168 |
R-free | 0.20240 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.008 |
RMSD bond angle | 1.084 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | MOLREP |
Refinement software | PHENIX (dev_777) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 20.000 |
High resolution limit [Å] | 1.700 |
Number of reflections | 63578 |
<I/σ(I)> | 17.94 |
Completeness [%] | 95.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 293 | HEPES, MgCl2, PEG 4000, pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K |