3AQF
Crystal structure of the human CRLR/RAMP2 extracellular complex
Experimental procedure
Experimental method | MAD |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL26B2 |
Synchrotron site | SPring-8 |
Beamline | BL26B2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2009-07-17 |
Detector | RIGAKU JUPITER 210 |
Wavelength(s) | 0.9790, 0.9793, 0.9640 |
Spacegroup name | P 41 21 2 |
Unit cell lengths | 55.424, 55.424, 119.391 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 40.620 - 2.600 |
Rwork | 0.222 |
R-free | 0.28210 |
Structure solution method | MAD |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | SOLVE |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.690 |
High resolution limit [Å] | 2.600 | 2.600 |
Number of reflections | 6157 | |
<I/σ(I)> | 20.3 | 5.9 |
Completeness [%] | 99.1 | 91.8 |
Redundancy | 11.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 293 | 0.1M bis-Tris, 30 % (v/v) PEG MME 550, 0.05M Calcium Chloride, pH 6.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K |