3AOE
Crystal structure of hetero-hexameric glutamate dehydrogenase from Thermus thermophilus (Leu bound form)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PHOTON FACTORY BEAMLINE AR-NW12A |
Synchrotron site | Photon Factory |
Beamline | AR-NW12A |
Temperature [K] | 95 |
Detector technology | CCD |
Collection date | 2010-05-29 |
Detector | ADSC QUANTUM 210 |
Wavelength(s) | 1.0 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 121.986, 146.093, 158.284 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 30.220 - 2.600 |
R-factor | 0.19594 |
Rwork | 0.193 |
R-free | 0.25961 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1b26 |
RMSD bond length | 0.012 |
RMSD bond angle | 1.464 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | REFMAC (5.5.0110) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.220 | 2.640 |
High resolution limit [Å] | 2.600 | 2.600 |
Number of reflections | 87634 | |
Completeness [%] | 99.9 | 100 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5.6 | 293 | 0.1M sodium citrate tribasic (pH5.6), 1.0M ammonium phosphate monobasic, VAPOR DIFFUSION, HANGING DROP, temperature 293K |