3ANW

A protein complex essential initiation of DNA replication

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL38B1
Synchrotron site	SPring-8
Beamline	BL38B1
Temperature [K]	100
Detector technology	CCD
Collection date	2010-07-05
Detector	ADSC QUANTUM 210
Wavelength(s)	1.0000
Spacegroup name	P 41 21 2
Unit cell lengths	76.343, 76.343, 181.333
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	37.350 - 2.650
R-factor	0.262
Rwork	0.262
R-free	0.31000
Structure solution method	MIR
RMSD bond length	0.010
RMSD bond angle	1.400
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	SHARP
Refinement software	CNS (1.2)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	37.350	2.740
High resolution limit [Å]	2.650	2.650
Rmerge	0.043	0.388
Number of reflections	16278
<I/σ(I)>	17.6	9.9
Completeness [%]	99.4	100
Redundancy	14	14

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	293	0.1M HEPES, 0.2M magnesium chloride, 24% PEG 400, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K