3ALQ
Crystal structure of TNF-TNFR2 complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL41XU |
| Synchrotron site | SPring-8 |
| Beamline | BL41XU |
| Temperature [K] | 95 |
| Detector technology | CCD |
| Collection date | 2006-03-25 |
| Detector | RAYONIX MX225HE |
| Wavelength(s) | 1.000 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 74.470, 117.356, 246.835 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 49.960 - 3.000 |
| R-factor | 0.2199 |
| Rwork | 0.213 |
| R-free | 0.28150 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2e7a |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.759 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | AMoRE |
| Refinement software | PHENIX (1.5_2) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.060 |
| High resolution limit [Å] | 2.950 | 2.950 |
| Rmerge | 0.183 | 0.603 |
| Number of reflections | 45945 | |
| <I/σ(I)> | 10.75 | 1.51 |
| Completeness [%] | 98.9 | 89.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | VAPOR DIFFUSION, HANGING DROP |
