3AJV

Splicing endonuclease from Aeropyrum pernix

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE BL-17A
Synchrotron site	Photon Factory
Beamline	BL-17A
Temperature [K]	100
Detector technology	CCD
Collection date	2008-12-13
Detector	ADSC QUANTUM 270
Wavelength(s)	1
Spacegroup name	H 3
Unit cell lengths	95.334, 95.334, 253.819
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	30.000 - 1.700
R-factor	0.21809
Rwork	0.218
R-free	0.22893
Structure solution method	SIRAS
RMSD bond length	0.014
RMSD bond angle	1.505
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	SHELXCD
Refinement software	REFMAC (5.5.0066)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.730
High resolution limit [Å]	1.700	1.700
Rmerge	0.072	0.346
Number of reflections	90770
<I/σ(I)>	14	3.1
Completeness [%]	98.5	85.1
Redundancy	4.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	4.2	295	20mM Tris-HCl (pH 8.0), 750mM KCl, 10% (v/v) glycerol, 0.2M NaCl, 0.1M phosphate-citrate (pH 4.2), 10% (w/v) PEG3000, VAPOR DIFFUSION, SITTING DROP, temperature 295K
1	VAPOR DIFFUSION, SITTING DROP	4.2	295	20mM Tris-HCl (pH 8.0), 750mM KCl, 10% (v/v) glycerol, 0.2M NaCl, 0.1M phosphate-citrate (pH 4.2), 10% (w/v) PEG3000, VAPOR DIFFUSION, SITTING DROP, temperature 295K