3AJI

Structure of Gankyrin-S6ATPase photo-cross-linked site-specifically, and incoporated by genetic code expansion

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL41XU
Synchrotron site	SPring-8
Beamline	BL41XU
Temperature [K]	100
Detector technology	CCD
Collection date	2008-12-15
Detector	RAYONIX MX225HE
Spacegroup name	H 3
Unit cell lengths	103.164, 103.164, 154.993
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	42.930 - 2.050
R-factor	0.171
Rwork	0.171
R-free	0.22900
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2dvw
RMSD bond length	0.032
RMSD bond angle	2.800
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	MOLREP
Refinement software	CNS (1.2)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.180
High resolution limit [Å]	2.050	2.050
Number of reflections	38604
<I/σ(I)>	20.7	3.2
Completeness [%]	100.0	99.9
Redundancy	4.2	4.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.5	298	31% polyethylene glycol 4000, 0.26M MgCl2, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 298.0K
1	VAPOR DIFFUSION, HANGING DROP	8.5	298	31% polyethylene glycol 4000, 0.26M MgCl2, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 298.0K